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{{/_source.additionalInfo}}Anti-RFP | Red flourescent protein (mRFP, mCherry, tdTomato, mScarlet)
(Cat#: AS24 5006)
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Description
| Immunogen: |
Recombinant protein produced in E.coli corresponding to full lenght mRFP. The sequence used for immunization is also found in other red flourescent proteins like: mCherry/tdTomato/mScarlet and others. |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Purity: | Antigen affinity purified serum, in PBS pH 7.4 |
| Format: | Lyophilized |
| Quantity: | 50 µg |
| Reconstitution: | For reconstitution, add 50 µl, of sterile or deionized water. |
| Storage: | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| Tested applications: | Immunohistochemistry (IHC) |
| Recommended dilution: | 1: 200 (IHC) |
| Expected | apparent MW: | Depends upon fusion partner |
- Confirmed reactivity: RFP
- Predicted reactivity: mRFP, mCherry, tdTomato, mScarlet
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Red fluorescent protein (RFP) once excited is fluorescing red-orange light. The mass of RFP is approximately 25.9 kDa and its excitation maximum is 558 nm and emission maximum is 583 nm. Improved variants of RFP include so called mFruits variants: mCherry, mOrange, mRaspberry.